FastQCFastQC Report
Thu 25 Jan 2018
HWNG7BGX3_n01_ko1060_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWNG7BGX3_n01_ko1060_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11609221
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA606450.5223864719260664TruSeq Adapter, Index 18 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG76300.057.9210148
CGTATGC77350.057.22395346
CGTCCGC77000.057.21671333
CCGCACA77800.056.4061636
TCCGCAC80000.055.1137435
GTCCGCA81550.053.98206334
CGCACAT81050.053.80358537
ACGTCCG82250.053.64824732
GTATGCC83050.053.42110447
ATGCCGT83100.053.222949
TCGTATG85450.051.63807345
CTCGTAT89300.048.90998544
AGTCACG93400.047.6131528
GTCACGT93800.047.37312329
TGCCGTC95100.046.57962850
GCCGTCT95600.046.2277551
GCACATC97100.044.59100338
TCACGTC100300.044.33786430
CATCTCG98900.043.7790641
ACGTCTG104500.043.51234415
Produced by FastQC (version 0.11.2)