FastQCFastQC Report
Thu 25 Jan 2018
HWNG7BGX3_n01_ko060_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWNG7BGX3_n01_ko060_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7494894
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA208040.2775756401624893TruSeq Adapter, Index 15 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG31350.050.0705948
CGTATGC31750.049.11380446
GTATGCC36300.043.6229447
ATGCCGT36550.042.4750649
TCGTATG38300.040.8045245
GCCGTCT41050.038.5752251
TGCCGTC41100.038.1085950
CTCGTAT41150.037.2239144
ACGTCTG44100.036.92577715
GCACACG44900.036.34469611
CACACGT48450.033.53947412
ACACGTC49000.033.3742413
TCTCGTA47000.033.1779443
CACGTCT50950.032.23191514
GTCACAT51200.031.19833829
CACATGT52950.030.362531
ATGTCAG53900.029.63513434
CGTCTGA55650.029.57191316
AGCACAC56050.029.29873510
CAGTCAC57000.028.32640327
Produced by FastQC (version 0.11.2)