FastQCFastQC Report
Thu 25 Jan 2018
HWNG7BGX3_n01_ko060_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWNG7BGX3_n01_ko060_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8829590
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA89510.10137503553392627TruSeq Adapter, Index 14 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG19850.033.89127348
CGTATGC20250.033.05163646
GTATGCC24050.027.82934247
ATGCCGT25850.026.69213149
TCCGTAT25400.026.21484237
TCGTATG26300.025.71071245
CGTATCT27650.023.707339
TATCTCG28700.023.20045941
CTCGTAT29000.023.19799244
CCGTATC28300.023.16278538
TTCCGTA30300.022.65852436
GCACACG35600.021.12614611
GCCGTCT31650.021.03753951
ACGTCTG35700.020.97033115
TGCCGTC34150.019.80052650
CACACGT39100.019.49987212
ACACGTC38750.019.40890313
GTTCCGT36200.018.96577535
AGTTCCG37000.018.64905434
CACGTCT40500.018.4847514
Produced by FastQC (version 0.11.2)