FastQCFastQC Report
Thu 25 Jan 2018
HWNG7BGX3_n01_ko0240_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWNG7BGX3_n01_ko0240_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11796719
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG124540.10557172718956855RNA PCR Primer, Index 37 (100% over 43bp)
CCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGGTCG123580.10475794159376009No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG23150.039.93880546
CGTATGC23950.038.31728744
GTATGCC26950.034.30735845
GTGTGCG28000.033.1440438
ATGCCGT30150.031.35253347
TCGTATG29500.031.22539143
CGGAATA33900.027.47831334
CTCGTAT34650.026.68395242
TATCTCG35200.026.65928639
TGTGTGC37900.025.5789097
CGCGTAT13700.024.6815241
GCCAGTA29000.024.509571
GCCGTCT38500.024.10486249
GTGCGAG38350.023.83910410
TGCGAGT40400.022.97102211
CTGACAT47200.022.8075731
GCACACG42650.022.56807911
TGTGCGA41800.022.4493549
ACGTCTG44000.022.11115315
CGGGCGC29900.021.57671515
Produced by FastQC (version 0.11.2)