FastQCFastQC Report
Thu 25 Jan 2018
HWNG7BGX3_n01_kcl_control_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWNG7BGX3_n01_kcl_control_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9934042
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGGTCG120150.1209477471506563No Hit
CGGCAATCGGGCGGCGGGCGCATGCGTCGCTTCTAGCCCGGATTCTGACTTAGAGGCGTTCAGTCATAATCCAGC116490.11726344623870122No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC18700.040.0340644
TATGCCG19750.036.68290346
GTATGCC22450.033.3468645
TCGTATG22050.033.01302743
GGCGGGC28250.031.01939613
ATGCCGT23150.030.99745847
GTGTGCG23200.028.6999288
CGGGCGC31100.028.17678315
GCCAGTA26850.027.7574791
GCGGGCG33000.026.5543514
CTCGTAT27450.026.14175242
CGGCAAT38400.025.7882121
ACGTCTG33150.024.66510215
GCCGTCT29650.024.5511349
ACTCCCG32400.024.06536332
GCACACG34500.023.79946711
GGCGCAT39200.022.4425517
TGTGTGC31050.022.3329457
GTGCGAG30050.022.27237910
GCAATCG43050.021.879213
Produced by FastQC (version 0.11.2)