FastQCFastQC Report
Thu 25 Jan 2018
HWNG7BGX3_n01_kcl_control_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWNG7BGX3_n01_kcl_control_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8590879
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCATTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG154350.1796672959775129RNA PCR Primer, Index 45 (100% over 43bp)
CTGACATGTGTGCGAGTCAACGGGTGAGTAAACCCGTAAGGCGCAAGGAAGCTGATTGGCGGGATCCCTCGCGGG105290.1225602176447835No Hit
CCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGGTCG103240.12017396590034617No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCACAAGCCAGTTATCCCTGTGGTAACT96700.1125612408229705No Hit
CGGCAATCGGGCGGCGGGCGCATGCGTCGCTTCTAGCCCGGATTCTGACTTAGAGGCGTTCAGTCATAATCCAGC92260.1073929687520916No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC23850.049.75995644
TATGCCG24850.048.1740346
GTATGCC27000.044.97682645
TCGTATG27250.043.6779743
ATGCCGT28300.042.5450447
CTCGTAT28750.040.55914342
GTGTGCG24200.035.2130938
CATCTCG35050.033.7612539
GCCGTCT36400.032.9828249
GCACACG37550.032.80071611
ACGTCTG38100.032.32740415
CACACGT39550.031.14238412
CACGTCT42200.029.43185414
AGTCACT42800.028.77743128
CACTCAT42300.028.7094531
CTGACAT34250.028.2067381
TCTCGTA42150.028.15615341
TGTGTGC31100.027.844277
GTCACTC44450.027.5539729
CAGTCAC45350.027.53951827