Basic Statistics
Measure | Value |
---|---|
Filename | HWNG7BGX3_n01_kcl_control_1.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 8590879 |
Sequences flagged as poor quality | 0 |
Sequence length | 75 |
%GC | 47 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCATTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG | 15435 | 0.1796672959775129 | RNA PCR Primer, Index 45 (100% over 43bp) |
CTGACATGTGTGCGAGTCAACGGGTGAGTAAACCCGTAAGGCGCAAGGAAGCTGATTGGCGGGATCCCTCGCGGG | 10529 | 0.1225602176447835 | No Hit |
CCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGGTCG | 10324 | 0.12017396590034617 | No Hit |
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCACAAGCCAGTTATCCCTGTGGTAACT | 9670 | 0.1125612408229705 | No Hit |
CGGCAATCGGGCGGCGGGCGCATGCGTCGCTTCTAGCCCGGATTCTGACTTAGAGGCGTTCAGTCATAATCCAGC | 9226 | 0.1073929687520916 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CGTATGC | 2385 | 0.0 | 49.759956 | 44 |
TATGCCG | 2485 | 0.0 | 48.17403 | 46 |
GTATGCC | 2700 | 0.0 | 44.976826 | 45 |
TCGTATG | 2725 | 0.0 | 43.67797 | 43 |
ATGCCGT | 2830 | 0.0 | 42.54504 | 47 |
CTCGTAT | 2875 | 0.0 | 40.559143 | 42 |
GTGTGCG | 2420 | 0.0 | 35.213093 | 8 |
CATCTCG | 3505 | 0.0 | 33.76125 | 39 |
GCCGTCT | 3640 | 0.0 | 32.98282 | 49 |
GCACACG | 3755 | 0.0 | 32.800716 | 11 |
ACGTCTG | 3810 | 0.0 | 32.327404 | 15 |
CACACGT | 3955 | 0.0 | 31.142384 | 12 |
CACGTCT | 4220 | 0.0 | 29.431854 | 14 |
AGTCACT | 4280 | 0.0 | 28.777431 | 28 |
CACTCAT | 4230 | 0.0 | 28.70945 | 31 |
CTGACAT | 3425 | 0.0 | 28.206738 | 1 |
TCTCGTA | 4215 | 0.0 | 28.156153 | 41 |
TGTGTGC | 3110 | 0.0 | 27.84427 | 7 |
GTCACTC | 4445 | 0.0 | 27.55397 | 29 |
CAGTCAC | 4535 | 0.0 | 27.539518 | 27 |