FastQCFastQC Report
Thu 2 May 2024
HWN2YAFX5_n02_QG4628.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWN2YAFX5_n02_QG4628.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34
Sequences flagged as poor quality0
Sequence length147
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCAAGAAGGAATGTCGGTTTCCCCAGACGTGATCGCTAAAATCGAGGAG12.941176470588235No Hit
TAACGACGTTTCAGAACTCTGCGAGAATCCAGGGAAGAAGGGAAAGAAGG12.941176470588235No Hit
TGCCTCTCTCACCTTCTTCGGGTTTCTCGTATCAACCATCATGTCACACC12.941176470588235No Hit
AGTAAAAATGTCCCTCCGCGTTACCTACAGACGCCGTTTGTCTTACAACA12.941176470588235No Hit
TGCTCTCGGAGCACAGACTGGAAACCAGGCGGTGCAGATGGTCCGAGCCG12.941176470588235No Hit
GGGGGGGTACGCCGCCGACGAGTGCGAAATTGACGAGGAACACACAATCC12.941176470588235No Hit
TGATTTTTCTAACAGAATGAGCTTCTCATCCCCCTTTTTCGCTTCGTTTG12.941176470588235No Hit
TGGATCATCGAGCAGGAGATCTAGTCTGTCCAGCATGCGGCTTGGTCGTC12.941176470588235No Hit
TGTCTATTCCACCGTCATGCGTCCGTTCATCCTGCTCGCACTCCTCTTCT12.941176470588235No Hit
GAGGTGGTTTAGCAGTCTGGTTAGAAAGATCTAATCATAAAGACATTGGA12.941176470588235No Hit
TCAGGAAACGACGAGGGATCTGCTGAGGAAACGAGCACCGAGGCATCTGG12.941176470588235No Hit
AGTCAATGGTCTGCAAGAACTATACTCGTCCACACAAGAAGACTCATCGA12.941176470588235No Hit
AGAAAAATCCGAAAGGCGACAGTGAAGGATGAAATGGTTGAGAAAAACGA12.941176470588235No Hit
GGCAGGAAAGGAGACCTCTGAGACAGAGAAATGAGTCCAACGCAGATAGA12.941176470588235No Hit
ATCTATTTCTATTTTTAGAATCAAAGAGACTCTAAAATAAAACACCTAAC12.941176470588235No Hit
CGACACTTTGTGGATAAGAAATCTGCGGCGGAAAAAGTGAAAAAGACGGT12.941176470588235No Hit
TGTTGGAGCAACTGCCGAGATGGCAAACCACCGGATCAGTCAACCCATCT12.941176470588235No Hit
TGGAGAAGAAGCAGTTCAACCTCATCTGGATGAACAAGAACACCATCAAG12.941176470588235No Hit
TAATCAGTTTCCGAAATTAGTGGTTCTATCTCTGAATCGTTGCTATGGCA12.941176470588235No Hit
TCTGAGCAATACGAATCTCTCCGCCAGTCTGCTTGGCTTGCCGCTGGATC12.941176470588235No Hit
TGGAGGAGCAGATCCGCAAGGAGAACGCCTGGTGCGAGGACTAGATCCCC12.941176470588235No Hit
TGAAAGCGACAAAGTGGGTCAAACTCGAGAACGTCCTGAAAATGTCCTGT12.941176470588235No Hit
TAAAACAGCCAAGCTGTCAATCCCGCTGCTCACTTATCAGATGTTCGCCC12.941176470588235No Hit
GGCTCACGCGCAGAGAAAGACCAGAAAGCTCCGTGGTCACGTCTCGCACG12.941176470588235No Hit
GGATTTGGTAACACAACTACAGTAATCTTTTTCATTTTCCTAGTGTATAT12.941176470588235No Hit
TCGGTTTTTGAGCTTTTCCCTATTCCAAATTATTCATATTTAAGTATATT12.941176470588235No Hit
TGGCCTATCGTCTCATCGGCTACGCCGCAGTTTCCCTCTCGGCGGTCGGC12.941176470588235No Hit
TGTCATGACTGCTCTCATCAAGAGAAACACCACCATCCCAACCAAGACTG12.941176470588235No Hit
TCAGATGAGAAGTATTATCAAAAGCATTGGGAAAGACTGCCGATTTAGCA12.941176470588235No Hit
TGGACTCTTGTCAGGGAAGATGTCGAAAACAGGCGGAACAGGAAATGCGA12.941176470588235No Hit
TAATAAAAAATCAAAACCAAAAAACAAGAAAAAACATATAAAAGATTTAA12.941176470588235No Hit
TGTACCAAGGAAGCTGTCATGGAAATTCACGCTAGTCGTAAAATGGCCCA12.941176470588235No Hit
AAAGACCCATCTTCAAAAGAGGACAACTACAGCAGAGAATGATTTTGAAC12.941176470588235No Hit
TAAGTCTTTCAGTGTTCGGCGCTTCGGTTCCAGCACACACACTCTTCTTT12.941176470588235No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)