FastQCFastQC Report
Thu 2 May 2024
HWN2YAFX5_n01_QG4628.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWN2YAFX5_n01_QG4628.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCCATTAGTTTTCAAATAGCCAGCATAGCTGAAGTTAAAAGCGTGTTG12.941176470588235No Hit
AGGCATTTGACAGGGTGCGCCGGGAGACCCATGAAGTGATCGATGGTGGC12.941176470588235No Hit
AGCAGCACAATTGGCGATGGAGGACCATCCATTGACGAGTGGTTTGAAGG12.941176470588235No Hit
TCTCGAAAAAGTTCCATTTGGAGCCGCCCGACTTTAAACGGATGCCGGTT12.941176470588235No Hit
TCCCGTCCTCGGATTGACTCGAGTGGAGGCACCTGCCGGTTACGAGTGAG12.941176470588235No Hit
AGGTGGAGTCAGCCTTGCGGAAGAGTTGCATGAAGACGCGGACGTACTTC12.941176470588235No Hit
TGAAGGGGGAAAAAATGAAAAAGAAGAAGCTGGAAATATAGGTGGAGACC12.941176470588235No Hit
TGGATCGTTTCCACTGCGCTCGTTGGAGAACGATCTCCATTCTGTGCCGA12.941176470588235No Hit
AGCGTTGATTCCGTCGAATTGGACAGTGACGTCATCGGAGTCGACCTTGA12.941176470588235No Hit
AGTGAACATTGAGGAAACACATACTGGGCTTGTGGTTCAAATGGATCATA12.941176470588235No Hit
AGTCTTGTTTTCGTTGTCGATTCTGCGATTTTCCATCTCCATTCTGAAAA12.941176470588235No Hit
GGTGCTCTTCGCCTTCTCCTGTGCGTTTTTAATGGCTTTCTCTGAAGATG12.941176470588235No Hit
ACGGTGATTAGTTGTGATAATTATTCTAGCCCGTAACAATGAAGTTTGCA12.941176470588235No Hit
AGACAGAAATCTCGTATGCCGTGGATTCGTTGTAAATAAAATTGGGGGGG12.941176470588235No Hit
TGCTCTTCTCAATGATCTGTTGATTCTTCTGGCGAGTTCAGGCCCGGAGT12.941176470588235No Hit
AGTGCATTCTGGTTACTGTGGTTGTTGCCAACAAGACGCGCACTCCGACG12.941176470588235No Hit
TGGTGACTCAGTGACAAATGATCCGGTGAACTCTGGAACAACTCCATCGG12.941176470588235No Hit
AGTCCGGTAGTTGTGTCACCGATTCCTTGAATAACTTTAAAGTTAGGAGG12.941176470588235No Hit
AATCAGAGTTCTCGACGAGCGATTTGGGACTAAAAGTCAAGGGTGTTACC12.941176470588235No Hit
TCCTTGTCGTTGGTGATGGTGATCTTGTTCTGCTTTCCGGTGGACTTGTC12.941176470588235No Hit
TGTTCTGTCGGTGTGACGTCGCCGGAGCCAGATCCTTCAGCGTCCTTGGT12.941176470588235No Hit
TGCCTCCAGGAAAGAAGATGCTCTTCCTTCACAACGTTATACAGAGCTTC12.941176470588235No Hit
ACACAGGGAAAAACTAATTTTACAAAAAATAATTTTTTTTACTATTAAAA12.941176470588235No Hit
AGGCGGCGGTGGTAGACTGGACTTCGTACTGAACGTATGGTTGAGCATCC12.941176470588235No Hit
TGCTTGAAGAGAGTGTAAGCCTCAGCATCTGGGGCGTAGACTCCGACTCC12.941176470588235No Hit
GTTTTTTTTTTAAACAACATTTCATTGAAAACAAAACAACGATTTACTTT12.941176470588235No Hit
AGCAGTAATAACAGAATTATATAATTGACCATTACTAAGAAAAAATCCAG12.941176470588235No Hit
TCCCTGCTGCTTCTGCTTGTTCTTCTTGTTATTGTTTCCGTTCGACGCCT12.941176470588235No Hit
ACTCCGGTCCTCGTTCTCAAAGAAGTGGTGCAAGTAGAGGTTACACTCTT12.941176470588235No Hit
AGGGAGCGGAGGTTGCTGACGTAAGAGTAGACGAGCGGAAGGGTGATGCA12.941176470588235No Hit
TCGCGGTTTCTTGGTGGTGTTGCGTCCCGGGTGACCGAGGGCTTCGCCTT12.941176470588235No Hit
TGCCACTTTGATCTCGTTGTCTCTCACTGATTGATTCAACGCGTGATACA12.941176470588235No Hit
GGGGCGAGCTGGGGTGATTCCGTGAAGCTTGACTCCGGTGTCTCTGCACT12.941176470588235No Hit
TCTATTCTCAACATTGCGGCTCAGCTGTCTTTTCAACCGCGACCGATCAC12.941176470588235No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)