FastQCFastQC Report
Fri 14 May 2021
HWMWVAFX2_n02_2456.dk.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWMWVAFX2_n02_2456.dk.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6075844
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC1915833.1531915565969104Illumina Single End PCR Primer 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG433360.7132507022892622No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATATCGGTGGTCGCC236250.38883486804467Illumina Single End PCR Primer 1 (98% over 50bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)