FastQCFastQC Report
Fri 14 May 2021
HWMWVAFX2_n01_2456.dk.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWMWVAFX2_n01_2456.dk.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6075844
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGGGGGTG797211.312097545624937TruSeq Adapter, Index 10 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGGGGTTG566430.9322655420382748TruSeq Adapter, Index 10 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGGGGGTT263590.4338327317159558TruSeq Adapter, Index 10 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGGGGTTT241480.3974427256526007TruSeq Adapter, Index 10 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGGGGGGG182610.3005508370524326TruSeq Adapter, Index 10 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGGGGATG142360.23430489657074804TruSeq Adapter, Index 10 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGGGTTTG119120.19605506658827973TruSeq Adapter, Index 10 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGCGGTTG77400.12738970914987285TruSeq Adapter, Index 10 (97% over 45bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGCGTATG71270.11730057585415295TruSeq Adapter, Index 10 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGCGGATG70040.11527616574750768TruSeq Adapter, Index 10 (97% over 45bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGGGTATG65960.10856104929619653TruSeq Adapter, Index 10 (97% over 42bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)