FastQCFastQC Report
Fri 14 May 2021
HWMWVAFX2_n01_2455.dk.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWMWVAFX2_n01_2455.dk.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8545385
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATGGGGGGTG208420.2438977295932249TruSeq Adapter, Index 20 (97% over 41bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATGGGGGTTG144150.1686875430422386TruSeq Adapter, Index 20 (97% over 41bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATGGGGGGTT132040.15451615111548514TruSeq Adapter, Index 20 (97% over 41bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATGGGGGTTT109760.12844359850375378TruSeq Adapter, Index 20 (97% over 41bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)