FastQCFastQC Report
Fri 14 May 2021
HWMWVAFX2_n01_2447.dk.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWMWVAFX2_n01_2447.dk.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6792043
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCATCTCGTATG180770.2661496695471451TruSeq Adapter, Index 18 (100% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCATCGGGGGTG170490.2510143118940796TruSeq Adapter, Index 18 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCATCGGGGTTG151660.22329069471438856TruSeq Adapter, Index 18 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCATCGCGTATG99740.1468483047000733TruSeq Adapter, Index 18 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCATCGGGGTTT96710.1423872022011639TruSeq Adapter, Index 18 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCATCGGGGGTT88540.13035842087572178TruSeq Adapter, Index 18 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCATCGCGGTTG74090.10908352612019684TruSeq Adapter, Index 18 (97% over 45bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCATCGCGTTTG68090.1002496597857228TruSeq Adapter, Index 18 (97% over 46bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)