FastQCFastQC Report
Sat 7 Dec 2019
HWMLGAFXY_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWMLGAFXY_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7939152
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1577711.9872525428408474No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGCAC94200.031.9578258
CTCCAGT60450.029.34570124-25
CGTCTGA69800.028.84615116-17
CACGTCT71500.028.01801514-15
CCAGTCA55150.027.50900326-27
CGGAAGA113950.026.5003284
AACTCCA76700.026.1757622-23
CACACGT77950.026.07723412-13
TCGGAAG115900.025.58363
TGAACTC82400.024.51442520-21
ATCGGAA125400.023.8637962
AGCACAC88800.023.71036110-11
CAGTCAC45250.023.5515626-27
GATCGGA122850.023.4382131
GAGCACA140050.021.8850179
ACGTCTG70550.021.24006714-15
CTGAACT72900.020.9036318-19
AAGAGCA151150.020.7291377
TCTGAAC101200.020.21244818-19
GAACTCC73400.019.92418520-21
Produced by FastQC (version 0.11.5)