FastQCFastQC Report
Sat 7 Dec 2019
HWMLGAFXY_n01_Xbp1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWMLGAFXY_n01_Xbp1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11213972
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGACTA28200.032.0033658
CATATGG35250.027.0870023
TATCGGG55700.023.15281538-39
TCACTAG98650.022.4617630-31
GCTTATC92700.021.80263536-37
TAGCTTA102250.021.48511134-35
CGCGCGT33550.020.6790051
ATCGGGG52900.020.20300940-41
AAGGGGG34450.020.1304272-73
TATGGGA47600.019.659995
CTTATCG85800.018.79497336-37
TACCCCC25900.018.61467612-13
ACTAGCT123650.018.20846732-33
TTCGTAC62150.017.579676
CTCATAT72650.017.5294571
TGGGACT55600.017.0008037
GTCCTTT66800.016.8593641
CACTAGC126800.016.76343330-31
CAGTCAC124600.016.6400826-27
CCTTTCG65450.016.6207223
Produced by FastQC (version 0.11.5)