FastQCFastQC Report
Sat 7 Dec 2019
HWMLGAFXY_n01_Pu7-D10C.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWMLGAFXY_n01_Pu7-D10C.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6261166
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCACC152150.033.1544328-29
ACCGATG155200.032.54878232-33
CCAGTCA158550.032.2954626-27
ATGTATC149500.031.05736236-37
CTCCAGT165450.031.00601624-25
GTATCGG71150.030.7094438-39
CGATGTA164950.030.50967634-35
AACTCCA172950.029.78502522-23
TCACCGA171200.029.56233230-31
TCTGAAC180700.028.61272818-19
ATCGGGG48050.028.56889240-41
TGAACTC180950.028.53382120-21
CGTCTGA185800.027.86568816-17
CACGTCT196850.026.33766714-15
AAGGGGG59800.026.13266472-73
GAGCACA229000.024.3097849
CACACGT218200.023.85859112-13
AGCACAC219750.023.59303710-11
AGAGCAC238650.023.4064128
ATCGGGT34800.021.36112240-41
Produced by FastQC (version 0.11.5)