FastQCFastQC Report
Sat 7 Dec 2019
HWMLGAFXY_n01_Pu6-D10B.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWMLGAFXY_n01_Pu6-D10B.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5898444
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATG621431.0535490376784113TruSeq Adapter, Index 1 (100% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC392060.6646837708385466TruSeq Adapter, Index 1 (100% over 50bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG166050.038.70341546-47
CGTATGC168450.038.56085644-45
CCGTCTT145150.037.48594350-51
CTCGTAT154950.036.52521542-43
TGCTTGA129650.033.5776558-59
CTTGAAA151200.032.67171560-61
GTCTTCT138500.032.63242752-53
TCTGCTT131500.032.4189656-57
TGCCGTC185450.032.28541648-49
ATCACGA217850.032.11704334-35
ATCTCGT173850.031.55714240-41
CTTCTGC140000.031.45155154-55
TCACATC235800.030.68944530-31
AGTCACA239000.030.34809928-29
ACATCAC238000.030.33590932-33
CACGATC190150.030.17595136-37
CCAGTCA252500.028.4903826-27
CTCCAGT260200.027.79331624-25
CGATCTC199800.027.7675638-39
TGAAAAA225450.027.70549262-63
Produced by FastQC (version 0.11.5)