FastQCFastQC Report
Sat 7 Dec 2019
HWMLGAFXY_n01_Pu3-D15C.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWMLGAFXY_n01_Pu3-D15C.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9186924
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC882110.9601799252938198TruSeq Adapter, Index 4 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATG380140.41378376483793705TruSeq Adapter, Index 4 (100% over 49bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG185450.040.39182346-47
CGTATGC191050.039.9661744-45
CTCGTAT182300.039.09650442-43
ACTGACC257000.037.6576432-33
AGTCACT272250.036.3246528-29
TGACCAA266350.035.3191934-35
ACCAATC227950.034.97601336-37
CCGTCTT161850.034.67445450-51
TCACTGA281250.034.6724930-31
GAGCACA405000.034.011959
CTCCAGT294550.033.8164524-25
CAATCTC234650.032.57047338-39
CCAGTCA307000.032.20524226-27
AACTCCA311700.032.03965422-23
TCTGAAC320550.031.2662118-19
TGAACTC330200.030.27334820-21
AGAGCAC473000.029.1724958
CGTCTGA345450.029.08129716-17
CACGTCT345600.029.0549314-15
CGGAAGA477350.028.8311254
Produced by FastQC (version 0.11.5)