FastQCFastQC Report
Sat 7 Dec 2019
HWMLGAFXY_n01_Fly-V3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWMLGAFXY_n01_Fly-V3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16236135
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTTTCGTACTAAAATATCATAATTTTTTAAAGATAGAAACCAACCTG188870.11632694603734202No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACGT106550.022.89155834-35
TACGTAA111500.021.85399636-37
ACCGTAC115050.021.24159432-33
GTACGTA103950.019.92279834-35
CCGTACG110650.018.69498432-33
AGGGGGG54500.018.04106774-75
GTACTAA125200.017.7174389
CGTACTA127050.017.5342248
TTCGTAC131650.017.3208396
CGTAATC119800.017.24728838-39
ACGTAAT122300.016.48692136-37
CCTTTCG136150.016.2947523
TAATCTC80250.015.86273740-41
AAAGGGG55300.015.718616572-73
AGTCACC161600.015.38734428-29
GTCCTTT147950.015.160261
TTTCGTA155600.014.89905555
CACCGTA140150.014.86157630-31
TCCTTTC155900.014.6269252
TATGCCG67800.014.25683348-49
Produced by FastQC (version 0.11.5)