FastQCFastQC Report
Sat 7 Dec 2019
HWMLGAFXY_n01_Fly-V2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWMLGAFXY_n01_Fly-V2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14046189
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTTTCGTACTAAAATATCATAATTTTTTAAAGATAGAAACCAACCTG179160.12755061177092236No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTGTAA112550.021.35471734-35
GTACTAA98950.020.9295359
CGTACTA100250.020.705518
TTCGTAC108600.019.2033796
AGTCACC129000.018.9629728-29
CCTTTCG113450.017.9218253
ACCTTGT141800.017.30146832-33
CAGTCAC124450.016.96546426-27
GTCCTTT120800.016.9149721
TGTAATG87250.016.63162436-37
CCAGTCA150250.016.2810226-27
TTTCGTA131700.016.0154885
TCCTTTC129450.015.7801972
AGGGGGG49500.015.73720272-73
CTTTCGT132600.015.2977424
CCTTGTA141500.015.15619432-33
AAGGGGG48100.014.96085472-73
TCGTACT150950.014.60077
GTCACCT149350.014.26415528-29
ACCCTAT66400.014.0924658
Produced by FastQC (version 0.11.5)