FastQCFastQC Report
Sat 7 Dec 2019
HWMLGAFXY_n01_Fly-V1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWMLGAFXY_n01_Fly-V1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7948730
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGC116950.14713042209258587TruSeq Adapter, Index 11 (100% over 50bp)
GTCCTTTCGTACTAAAATATCATAATTTTTTAAAGATAGAAACCAACCTG97680.12288755562209308No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACTA52600.019.1442858
TATGCCG41150.019.10368746-47
CGTATGC42300.018.9773444-45
GTACTAA54400.018.6854679
TTCGTAC56000.018.1537656
CCTTTCG60000.016.5476363
CTCGTAT45000.016.41371742-43
AGGGGGG24000.016.1300472-73
GTATGCC43100.015.53927944-45
AGTCACG69700.015.43563428-29
TCGTATG45200.015.13258542-43
GTCCTTT66250.015.062511
TCCTTTC70000.014.4551472
TTTCGTA72100.014.3635635
CTTTCGT71300.014.1915884
TCACGGC81300.013.55459430-31
CTACATC71800.013.03259436-37
GCTACAT68900.012.82279734-35
CACGGCT70800.012.78058530-31
AAAGGGG24500.012.69884670-71
Produced by FastQC (version 0.11.5)