FastQCFastQC Report
Sat 7 Dec 2019
HWMLGAFXY_n01_Fly-A43.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWMLGAFXY_n01_Fly-A43.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11300628
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTATCTCGTAT313360.27729432381987973TruSeq Adapter, Index 27 (97% over 44bp)
GTCCTTTCGTACTAAAATATCATAATTTTTTAAAGATAGAAACCAACCTG184070.16288475295355268No Hit
GAAAATTTTAAATGGCCGCAGTATTTTGACTGTGCAAAGGTAGCATAATC125470.11102922775619195No Hit
GTCTTCTCGTCTTTTAAATAAATTTTAGCTTTTTGACTAAAAAATAAAAT124230.10993194360525804No Hit
CGGCTACACCCAAAATTATATCTTAATCCAACATCGAGGTCGCAATCTTT117920.10434818312752177No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG71850.027.69977648-49
CGTATGC70850.026.98453946-47
TTATCTC86950.026.11243240-41
TATCTCG78000.025.91158540-41
CTTTATC139550.025.68138538-39
CTCGTAT72650.025.00833744-45
AGGGGGG61450.024.69664674-75
CCTTTAT139100.024.53514736-37
CGTACTA82300.024.4134738
ACATTCC161800.024.26349332-33
AGTCACA164200.024.01009828-29
TCGTATG66700.023.85656744-45
GTACTAA84500.023.7778539
AAAGGGG53000.023.74974872-73
TCCTTTA165700.023.52042236-37
GTATGCC79150.023.40467846-47
ATTCCTT173200.022.72132534-35
TTCGTAC91850.022.1879446
CAGTCAC161900.022.09210626-27
CCAGTCA181350.021.71330626-27
Produced by FastQC (version 0.11.5)