FastQCFastQC Report
Sat 7 Dec 2019
HWMLGAFXY_n01_Fly-A42.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWMLGAFXY_n01_Fly-A42.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8104888
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTAT1632342.0140191943429695TruSeq Adapter, Index 25 (97% over 44bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTA225670.27843691362545664TruSeq Adapter, Index 25 (97% over 44bp)
GTCCTTTCGTACTAAAATATCATAATTTTTTAAAGATAGAAACCAACCTG120940.14921859500094264No Hit
CTGGAATGAATGGTTGGACGAAATATTAACTGTTTCATTTAAAATTTTTA108390.133734112056823No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG104860.12937871565899492No Hit
CAAAATTATATCTTAATCCAACATCGAGGTCGCAATCTTTTTTATCGATA104000.12831762758473653No Hit
GTCTTCTCGTCTTTTAAATAAATTTTAGCTTTTTGACTAAAAAATAAAAT93530.1153994971923116No Hit
CTCGTCTTTTAAATAAATTTTAGCTTTTTGACTAAAAAATAAAATTCTAT87870.10841605707568075No Hit
CGGCTACACCCAAAATTATATCTTAATCCAACATCGAGGTCGCAATCTTT86460.10667636616323384No Hit
CAAAAACATGTCTTTTTGAATTATATATAAAGTCTAACCTGCCCACTGAA84900.1047516017494628No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG243900.040.7318148-49
CGTATGC245050.040.67634646-47
CTCGTAT243850.039.5811744-45
CCGTCTT242450.037.85058652-53
TGCCGTC261650.037.21523750-51
CTTGAAA227700.036.8398662-63
ATATCTC265950.036.783240-41
TGCTTGA218950.035.7846260-61
TCGTATG245400.035.45998444-45
GTATGCC247750.035.1428146-47
ACTGATA297500.035.03775834-35
TATCTCG247300.034.46727840-41
GCCGTCT235400.034.1515150-51
ACACTGA307150.034.01426732-33
ATGCCGT255250.033.96133848-49
ATATATC293800.033.95120238-39
ATCTCGT289250.033.81198542-43
TGATATA309950.033.5767336-37
TCACACT317550.032.93766830-31
TCTCGTA260200.032.59419342-43
Produced by FastQC (version 0.11.5)