FastQCFastQC Report
Sat 7 Dec 2019
HWMLGAFXY_n01_Fly-A41.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWMLGAFXY_n01_Fly-A41.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14770254
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATATCTCGTAT632710.4283677179823719TruSeq Adapter, Index 7 (97% over 36bp)
GTCCTTTCGTACTAAAATATCATAATTTTTTAAAGATAGAAACCAACCTG316060.21398413324510193No Hit
CGGCTACACCCAAAATTATATCTTAATCCAACATCGAGGTCGCAATCTTT213800.14475038817883565No Hit
GTCTTCTCGTCTTTTAAATAAATTTTAGCTTTTTGACTAAAAAATAAAAT183870.12448668790665347No Hit
GAAAATTTTAAATGGCCGCAGTATTTTGACTGTGCAAAGGTAGCATAATC181980.12320708905886113No Hit
CAAAATTATATCTTAATCCAACATCGAGGTCGCAATCTTTTTTATCGATA155650.10538071992533102No Hit
CTCGTCTTTTAAATAAATTTTAGCTTTTTGACTAAAAAATAAAATTCTAT155030.10496095734034093No Hit
CAAAAACATGTCTTTTTGAATTATATATAAAGTCTAACCTGCCCACTGAA149470.10119663480397832No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC117250.030.52522746-47
TATGCCG118850.030.17423248-49
CTCGTAT120500.029.40629244-45
TATCTCG125200.026.55719440-41
TCGTATG118000.026.42659644-45
AGTCACG174100.025.79593328-29
ATATCTC147550.025.67319740-41
GTATGCC125200.025.49490746-47
GGATATC171850.024.57201238-39
AGGGGGG77250.023.24244574-75
TCACGAG193850.023.21677830-31
GATATCT152000.022.67160838-39
CGTACTA144800.022.6344018
GTGGATA200750.022.28865436-37
GTACTAA147450.022.1953969
ACGAGTG205700.021.879332-33
GTCACGA181200.021.82300428-29
TCTCGTA146150.021.66156642-43
GAGTGGA209850.021.6390134-35
CCTTTCG150300.021.5243993
Produced by FastQC (version 0.11.5)