FastQCFastQC Report
Sat 7 Dec 2019
HWMLGAFXY_n01_25D5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWMLGAFXY_n01_25D5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3597416
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATG265670.7385023027639839TruSeq Adapter, Index 9 (100% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC165050.45880153977188065TruSeq Adapter, Index 9 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC66550.037.3643444-45
CTCGTAT64250.035.89259342-43
TATGCCG69850.035.599146-47
CCGTCTT65150.034.66768650-51
CTTGAAA70850.028.9622760-61
GTCTTCT68950.028.89923952-53
AGATCTC78750.028.8615838-39
CTTCTGC68300.028.5135954-55
AGTCACG94650.028.10322228-29
ATCTCGT80250.027.96697440-41
GATCAGA93100.027.78029834-35
TGCTTGA69250.027.539458-59
TCACGAT98750.027.00855430-31
TCTGCTT69700.026.78233756-57
ACGATCA100400.026.30448332-33
TGCCGTC92350.026.0771348-49
TCAGATC91050.025.5625936-37
CCAGTCA110700.024.09326-27
CTCCAGT111550.023.86683324-25
TGAAAAA101950.023.73808562-63
Produced by FastQC (version 0.11.5)