FastQCFastQC Report
Sat 7 Dec 2019
HWMLGAFXY_n01_25D1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWMLGAFXY_n01_25D1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6366046
Sequences flagged as poor quality0
Sequence length101
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGGGGTTGG470040.7383547024322477TruSeq Adapter, Index 8 (97% over 42bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGGGGGTGG443140.6960992741805511TruSeq Adapter, Index 8 (97% over 42bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGGGGTTTG438330.6885435637756937TruSeq Adapter, Index 8 (97% over 42bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGGGGGTTG326870.5134584324398536TruSeq Adapter, Index 8 (97% over 42bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT118050.18543692584062382No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGGGGATGG110360.1733572141954362TruSeq Adapter, Index 8 (97% over 42bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGGGGGGGG100080.15720904310147932TruSeq Adapter, Index 8 (97% over 42bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGGGGATTG89620.14077812193000178TruSeq Adapter, Index 8 (97% over 42bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGGGTTTTG89070.13991416335979978TruSeq Adapter, Index 8 (97% over 42bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGGGTTTGG80280.12610653457420823TruSeq Adapter, Index 8 (97% over 42bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGGGGTTGC63830.10026631915634916TruSeq Adapter, Index 8 (97% over 42bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA732300.064.804959
CGGAAGA783650.060.870644
TCGGAAG787300.060.497923
AAGAGCA794850.059.9203077
AGAGCAC794700.059.8479358
GATCGGA798500.058.927831
GGAAGAG816450.058.442225
ATCGGAA821700.057.9554672
GAAGAGC825150.057.636046
ACACTTG595900.042.9240332-33
ACTTGAA594650.042.46310434-35
TCACACT606000.042.3889130-31
AGTCACA612400.042.0351128-29
TTGAATC535750.041.59910636-37
ATCGGGG337650.041.17640-41
CTCCAGT630400.040.99310324-25
TGAATCG453000.040.4583836-37
AACTCCA652650.039.7520522-23
AATCGGG379600.039.5411338-39
TCTGAAC662400.039.44659418-19
Produced by FastQC (version 0.11.5)