FastQCFastQC Report
Sat 7 Dec 2019
HWMLGAFXY_n01_20D3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWMLGAFXY_n01_20D3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7568524
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCGGGGTTGG127220.1680908985688623TruSeq Adapter, Index 7 (97% over 42bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCGGGGTTTG99710.13174299242494308TruSeq Adapter, Index 7 (97% over 42bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCGGGGGTGG77490.10238456005424572TruSeq Adapter, Index 7 (97% over 42bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA395900.046.743479
AGAGCAC408550.045.3310248
AAGAGCA432050.043.01937
CGGAAGA447400.041.5599944
TCGGAAG457200.040.5652583
GAAGAGC465400.040.2384156
GGAAGAG463850.040.1373145
ATCGGAA472450.039.266442
GATCGGA486600.037.7650831
CAGATCA281900.036.95141634-35
CCAGTCA297900.035.75604226-27
TCACCAG301550.035.34687830-31
CTCCAGT304550.035.27942724-25
ACCAGAT306150.034.7459632-33
AGTCACC311800.034.1468128-29
GATCATC229100.033.13135536-37
AACTCCA332700.032.48000722-23
ATCGGGG98900.032.0104240-41
CGTCTGA343700.031.64088616-17
TCTGAAC344700.031.63177118-19
Produced by FastQC (version 0.11.5)