FastQCFastQC Report
Sat 7 Dec 2019
HWMLGAFXY_n01_20D1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWMLGAFXY_n01_20D1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12162471
Sequences flagged as poor quality0
Sequence length101
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC515400.4237625725890734TruSeq Adapter, Index 6 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATG196170.16129123761117292TruSeq Adapter, Index 6 (100% over 49bp)
CCCTAGTCCGTGCCAGTTCTGAGCTGAGCGTTGAATGGCGGCCGAAGAGG175910.14463343838599904No Hit
CTTTGTTTTAATTAGACAGTCGGATTCCCCTAGTCCGTGCCAGTTCTGAG158690.130475131245945No Hit
CTGGCAGTGTCCTCGAATCGGATCACGCGGGAGTATTAACGGCGATCGGC157260.1292993833243261No Hit
CCCCAGTCAAACTCCCCGCCTGGCAGTGTCCTCGAATCGGATCACGCGGG148260.12189957123022123No Hit
CTCTAAGAAGATTTGTTTGTACGTTGGTAGTAAAAACCCGCCGGCCGAAG146400.1203702767307729No Hit
CTTGTTACGACTTTTACTTCCTCTAAATGATCAAGTTTGGTCATCTTCCC134420.11052030463217549No Hit
CCCCCATCTGTCCCTATTAATCATTACCTCGGGGTTCCGAAAACCAATAA130300.10713283509576302No Hit
CGGGAAGCGGGTCTGGCTGACGTGCCGGGCCTGGGCGAGGTGAACGTCCA130080.10695195080012934No Hit
CTCGGGGTTCCGAAAACCAATAAAATAGAACCGAGGTCCTATTCCATTAT127650.10495400153472104No Hit
GCCAGTTCTGAGCTGAGCGTTGAATGGCGGCCGAAGAGGACGACCACGAC122970.10110609924578648No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC92150.040.59234244-45
TATGCCG92600.040.42072746-47
CTCGTAT100200.039.1088642-43
GCCAATA152200.037.07581334-35
TGTTACG38000.036.7539833
CAATATC149450.036.58207336-37
ACGCCAA155450.036.43816832-33
ATATCTC130050.035.7204938-39
AGTCACG162450.035.0142428-29
CTTGTTA42500.032.7603031
GTTACGA43750.032.249214
CTCCAGT180900.031.67945124-25
TTACGAC45800.030.5982955
TCGTATG92150.029.89658242-43
GTATGCC94050.029.67139244-45
TACGACT47800.029.4174166
GAGCACA285350.029.280399
TGAAAAA135250.028.92112762-63
ATGCCGT89350.027.9361846-47
CCAATAT151000.027.115634-35
Produced by FastQC (version 0.11.5)