FastQCFastQC Report
Sat 7 Dec 2019
HWMLGAFXY_n01_15D3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWMLGAFXY_n01_15D3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11387236
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAGTCA209500.031.18643626-27
ACAGTGA206450.031.07197834-35
AGTCACA212100.030.84893228-29
CTCCAGT215250.030.55195424-25
ACACAGT214300.030.47682432-33
AGTGATG173250.029.95279736-37
AACTCCA232850.028.36507622-23
TGAACTC239250.027.73535220-21
TCTGAAC240100.027.67672518-19
TCACACA239800.027.49346530-31
GAGCACA326000.027.4797829
CGTCTGA250000.026.59022916-17
AGAGCAC349250.025.609628
CACGTCT269200.024.66728414-15
AAGGGGG87100.023.88609572-73
AAGAGCA391000.022.9601257
CACAGTG198950.022.29934732-33
AGCACAC309200.021.65283610-11
CACACGT313250.021.32739812-13
GTCACAC211000.021.14966628-29
Produced by FastQC (version 0.11.5)