FastQCFastQC Report
Sat 7 Dec 2019
HWMLGAFXY_n01_10D2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWMLGAFXY_n01_10D2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10295363
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTTAGG126100.030.68069632-33
TTAGGCA131450.029.16098234-35
TCACTTA142500.027.3163930-31
AGTCACT147900.026.3511528-29
AGGCATC82900.024.38002836-37
CTCCAGT163800.023.74975824-25
AAGGGGG55700.022.0015772-73
AGGCATG72250.021.56379536-37
AACTCCA182000.021.54442222-23
CCAGTCA181050.021.51317626-27
GCATGGG42750.021.33311738-39
TCTGAAC186850.021.1631518-19
TGAACTC189350.020.7833920-21
CGTCTGA195500.020.14173916-17
CACGTCT208250.018.90857114-15
TAGGCAT113650.017.36563936-37
CACACGT241000.016.38831912-13
AGCACAC241350.016.36455310-11
CTTAGGC136400.015.722898534-35
CACTTAG142200.015.36552632-33
Produced by FastQC (version 0.11.5)