FastQCFastQC Report
Thu 2 Aug 2018
HWM3CBGX5_n03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWM3CBGX5_n03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences499812088
Sequences flagged as poor quality0
Sequence length99
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG30550050.6112307151722989No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCGGTCGAGTGCCTCACAG24929070.4987688493040208No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCGGTCGTGCATTCCGCA23669520.47356837836222965No Hit
GTGCCAACCCCCCTGGCCCCCCCAAAAGGGGGGTGGTGCTGGTGCAATTG8126940.16259990894818055No Hit
GCTAAATAAACAAGAAAGCAAAATCAACAACCGCAAAAGTCTAAGACATG6294420.12593572966966737No Hit
GCAGTGGTATCAACGCAGAGTACATGGGGGGCGGTCGTGCATTCCGCAGC5089210.10182246732696068No Hit
GGTGCATATGGTGCCAACCCCCCTGGCCCCCCCAAAAGGGGGGTGGTGCT5066120.10136049370618662No Hit
GCAGTGGTATCAACGCAGAGTACATGGGGGCGGTCGAGTGCCTCACAGTG5030180.10064142346233132No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCAGTG16756750.070.79162
AAGCAGT17130150.069.447431
CAGTGGT18381750.064.451794
AGTGGTA18744300.063.2702645
GCAGTGG19177050.061.911343
GTGGTAT19767500.060.003626
GGTATCA20099200.059.0657658
TGGTATC20540650.057.7083937
GTATCAA25815650.046.020219
CATGGGG14042450.033.86261724-25
AGAGTAC19685600.033.47875218-19
GCAGAGT19886350.033.1832816-17
TACATGG20052500.032.64525222-23
CAACGCA20776900.031.83435812-13
ACGCAGA20853700.031.72776614-15
ATCAACG21177200.031.27544610-11
AGTACAT21709150.030.33191120-21
CGCAGAG19931300.029.94796416-17
CAGAGTA20083000.029.63365218-19
CCTCACT5044400.029.48912272-73
Produced by FastQC (version 0.11.2)