FastQCFastQC Report
Thu 2 Aug 2018
HWM3CBGX5_n02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWM3CBGX5_n02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences499812088
Sequences flagged as poor quality0
Sequence length8
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGCATCA388838287.779689393986805No Hit
CCTGTGCG353388797.070433038426234No Hit
CAACGCCT348988766.982399353254537No Hit
GGACACGT333544166.673391220582084No Hit
CTAGACCT327478916.552040614111758No Hit
TACTCTTC317895006.360290349760408No Hit
TGTGCGGG317584396.354075814188792No Hit
AGTTCGGC311527376.232889869602353No Hit
GAGATGAC310237636.207085371652716No Hit
ACTTCATA283909195.680318600057548No Hit
TCCGGAAG283616435.674461198705543No Hit
ATGAGAAA253262805.067160360475316No Hit
ACCATAAC246399384.9298403523205705No Hit
TGCCGTGG229006474.581851369709169No Hit
GTAATCTT226361404.528930080618618No Hit
GGGGGGGG202299224.04750554972572No Hit
GTGTATTA124902472.4989885798840463No Hit
CTAGCCCT5088680.10181186334172854No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)