FastQCFastQC Report
Thu 2 Aug 2018
HWM3CBGX5_n01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWM3CBGX5_n01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences499812088
Sequences flagged as poor quality0
Sequence length27
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACTAAC1339100.017.802441
CTGCGGA1100150.017.7817751
CAATGGA1406400.017.7447878
GGGACCT1156850.017.5894891
GGGAATG1176450.017.531291
CTCGAAA1237300.017.3653141
ACGAGGA1202150.017.3417591
GAATGAA1321050.017.291031
GACCTGG1390100.017.2876011
ACTGATG1242700.017.263651
GCGCGAT973400.017.2588621
TGACTAG1308300.017.2090171
TGACGGC1251150.017.1260661
TGGGCGT1087750.017.1152941
CGCTGGA1140450.017.1055221
ATAACGC1215450.017.0949541
GATGAAA1361600.017.0746631
CGTGAGC1078500.017.041951
GGCTCGA1245800.016.967721
GACTGCG1232250.016.9454351
Produced by FastQC (version 0.11.2)