FastQCFastQC Report
Fri 27 Jul 2018
HWLMWBGX5_n01_mb119.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWLMWBGX5_n01_mb119.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33839792
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCATCTCGTATGC1478050.4367786894198404TruSeq Adapter, Index 14 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCATGTCGTATGC395640.11691561224726203TruSeq Adapter, Index 14 (97% over 41bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG235900.061.85812843
CTCGTAT192800.061.0904642
CGTATGC254100.060.9380444
TCTCGTA194350.060.40558241
ATGCCGT248800.059.4630447
TATGCCG265900.058.7330946
TGCCGTC247250.058.58967648
ATCTCGT213950.057.6042140
CCGTCTT235650.057.47910750
GCCGTCT230300.057.4159549
CGTCTTC229900.055.4002251
CATCTCG226350.055.11468539
TCGCGTA17350.054.87417641
GTCGTAT63400.051.78559542
GTATGCC304800.051.74247745
CGCGTAT19300.051.68718742
CGTCTGA421400.051.05048416
ACGTCTG436200.049.38241215
TGTCGTA66100.049.3529441
CCGTCGT26150.049.25412450
Produced by FastQC (version 0.11.2)