FastQCFastQC Report
Fri 27 Jul 2018
HWLMWBGX5_n01_mb118.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWLMWBGX5_n01_mb118.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36370130
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAAATCTCGTATGC2528040.6950868748613216TruSeq Adapter, Index 13 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT317200.061.97112342
TCGTATG327250.061.85391243
TCTCGTA319850.061.72057341
CGTATGC330300.061.32336844
AATCTCG328600.060.73958239
ATGCCGT329850.060.46088847
TATGCCG337200.059.9324446
TGCCGTC330700.059.4902248
ATCTCGT337800.058.91808740
GCCGTCT322200.058.89786549
CCGTCTT325150.058.72932450
CGTCTTC325650.056.8441451
GTATGCC384900.053.0777845
CGTCTGA457800.049.338516
ACGTCTG476200.047.51267615
ACACGTC496350.045.7244113
GTCAAAT478050.044.99729535
GCTTGAA407800.044.645159
CACGTCT507600.044.6286514
CAGTCAA500050.044.20944633
Produced by FastQC (version 0.11.2)