FastQCFastQC Report
Fri 27 Jul 2018
HWLMWBGX5_n01_mb117.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWLMWBGX5_n01_mb117.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31687740
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGC5736011.8101669604711477TruSeq Adapter, Index 11 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG684700.064.3799643
CTCGTAT678800.064.3572742
TCTCGTA677000.064.1101141
CGTATGC690750.064.0681344
ACGGCTA719350.063.786732
TATGCCG694450.063.68504746
ATGCCGT690900.063.61663447
CGGCTAC719150.063.21615633
TGCCGTC692100.062.96532448
GCCGTCT675450.062.68859549
ATCTCGT694600.062.63209540
CATCTCG695950.062.43121739
CCGTCTT680900.062.40291250
TCACGGC737550.062.3382530
GTCACGG746400.061.82813629
CGTCTTC677300.061.45771451
AGTCACG758900.061.10025828
CACGGCT753350.060.97788631
GTATGCC739850.059.99479745
CGTCTGA800250.058.7595116
Produced by FastQC (version 0.11.2)