FastQCFastQC Report
Fri 27 Jul 2018
HWLMWBGX5_n01_mb116.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWLMWBGX5_n01_mb116.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32839076
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGC765800.23319779155783799TruSeq Adapter, Index 10 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGCGTATGC447860.1363802075308087TruSeq Adapter, Index 10 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGGGGTTGG401430.12224156367858828TruSeq Adapter, Index 10 (97% over 42bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGCG212900.065.7974439
CTTATCG575450.065.3360137
TCGCGTA87250.064.5884441
TTATCGG355150.064.5072838
TATCGGG349000.064.32963639
ATCGCGT138950.064.2109340
TTATCGC226350.063.60468738
CGCGTAT86800.063.5925642
CGTCTGA976300.062.1754916
GCTTATC927000.062.17418336
ACGTCTG986850.061.53161215
ACACGTC995000.061.17477813
TAGCTTA978800.060.6793234
ACTAGCT990050.060.437432
CACGTCT1006750.060.4194514
GTCACTA999100.060.1157529
ATCGGGT167800.059.92959640
ATCGGGG207350.059.82563840
AGCTTAT984150.059.7916435
CACTAGC1004100.059.70659331
Produced by FastQC (version 0.11.2)