FastQCFastQC Report
Fri 27 Jul 2018
HWLMWBGX5_n01_mb115.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWLMWBGX5_n01_mb115.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31588640
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC21784346.896257641987752TruSeq Adapter, Index 9 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATATCGTATGC343350.10869413814586511TruSeq Adapter, Index 9 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT2487400.065.3379542
TCTCGTA2468600.065.3267341
TCGTATG2557850.065.2291243
GATCTCG2452750.065.18552439
CGATCAG2564500.065.17733
CGTATGC2564150.065.1731644
ACGATCA2577400.065.0954932
ATGCCGT2562700.065.0773847
TATGCCG2565800.065.07040446
ATCTCGT2462100.064.97948540
CACGATC2582450.064.9183431
GTCACGA2587150.064.9086429
GCCGTCT2542100.064.8791549
TGCCGTC2562100.064.8726848
TCACGAT2588100.064.8453730
CCGTCTT2545550.064.8219850
CGTCTGA2639350.064.7427116
ACACGTC2665000.064.58848613
CGTCTTC2542650.064.5738151
AGTCACG2611300.064.47272528
Produced by FastQC (version 0.11.2)