FastQCFastQC Report
Fri 27 Jul 2018
HWLMWBGX5_n01_mb114.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWLMWBGX5_n01_mb114.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36020446
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAATCG212300.060.1157437
TCGCGTA23550.057.0740841
AATCGCG81700.056.7253539
AATCGGG138300.056.49152439
GAATCGG141950.056.0259238
ATCGCGT43750.055.5244540
CGCGTAT24000.054.9824242
GAATCGC91950.051.84912538
ATCGCGG42600.051.84687440
ATCGGGG105550.049.080240
CGTCTGA416400.047.56139816
ATCGGGT57350.046.08041440
ACGTCTG431500.045.94530515
AATCTCG69300.044.7014239
TTGAATC410400.044.54966736
ACACGTC455850.043.67500713
TCGCGTT22350.042.91129741
CACGTCT464050.042.89600414
GTCACAC465000.042.00096529
CTTGAAT465800.040.5067835
Produced by FastQC (version 0.11.2)