FastQCFastQC Report
Fri 27 Jul 2018
HWLMWBGX5_n01_mb113.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWLMWBGX5_n01_mb113.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28101666
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC642830.22875156227392357TruSeq Adapter, Index 3 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGTA20300.058.96870441
CGCGTAT20600.057.42957342
TCGTATG88900.057.12795343
TCTCGTA93350.057.1053941
CTCGTAT93350.056.8047242
CGTATGC102100.056.1177944
ATCGCGT28950.055.61643240
ATGCCGT98550.054.15987847
TATGCCG107850.054.09832446
CATCGCG40850.052.3543839
TGCCGTC102950.050.34935848
CCGTCTT100700.049.3199750
CATCTCG128600.049.10202839
CGTCTGA242250.049.09001516
ATCTCGT123850.048.666640
ACGTCTG246400.048.3906115
ACACGTC252450.047.188513
GCCGTCT98550.047.12830749
CACGTCT266600.044.69777314
CTTAGGC259450.044.62012533
Produced by FastQC (version 0.11.2)