FastQCFastQC Report
Fri 27 Jul 2018
HWLMWBGX5_n01_mb112.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWLMWBGX5_n01_mb112.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35786555
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC12019323.358613311619406TruSeq Adapter, Index 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT1374800.066.06515542
TCTCGTA1357350.066.04851541
TCGTATG1422250.065.9941243
CGATCTC1343700.065.8542438
CGTATGC1429400.065.8434644
ACGATCT1345400.065.7412937
CACGATC1348850.065.665736
ATGCCGT1428300.065.6646347
TATGCCG1431650.065.6579246
TCACGAT1399650.065.4667435
ATCACGA1423800.065.37382534
TGCCGTC1429200.065.2780748
CCGTCTT1415500.065.19527450
GCCGTCT1415200.065.169849
GATCTCG1363450.065.0920539
CATCACG1450350.064.9497433
CGTCTTC1409950.064.81951
ATCTCGT1374600.064.6391840
GTATGCC1492650.063.11555545
CGTCTGA1540600.062.66565316
Produced by FastQC (version 0.11.2)