FastQCFastQC Report
Fri 27 Jul 2018
HWLMWBGX5_n01_mb111.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWLMWBGX5_n01_mb111.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38763156
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC3510280.905571259471236TruSeq Adapter, Index 12 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATGTCGTATGC577770.14905133111452534TruSeq Adapter, Index 12 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGTA415450.065.04240441
TCGTATG469150.064.96802543
CTCGTAT412950.064.9100642
CGTATGC480100.063.95152344
ATGCCGT456200.063.80100647
AATCTCG447450.063.5376439
TATGCCG482700.063.243246
TGCCGTC460950.062.26251648
CCGTCTT449900.061.97182550
GCCGTCT440350.061.45569649
ATCTCGT466400.060.30174340
CGTCTTC435350.060.18403251
GTCGTAT85650.057.4173342
GTATGCC556100.055.8522945
CGTCTGA714350.055.5462216
GTAATCT526650.055.4654437
ACGTCTG724550.054.81213415
TGTCGTA91150.054.75967841
ACACGTC739150.053.77639413
CCTTGTA725650.053.59767533
Produced by FastQC (version 0.11.2)