FastQCFastQC Report
Fri 27 Jul 2018
HWLMWBGX5_n01_mb110.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWLMWBGX5_n01_mb110.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28427481
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC645760.2271604719391071TruSeq Adapter, Index 7 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGTA37500.063.84412441
CATCGCG91800.063.2192639
ATCGCGT63450.063.05371540
CGCGTAT38100.062.37880742
ATCATCG175850.059.87655337
TCTCGTA100500.059.3471241
CGTATGC110000.058.73695444
CTCGTAT101800.058.07298742
TCGTATG96200.057.99638743
TATGCCG124200.057.12164746
CGTCTGA417150.056.8979616
ATGCCGT106200.055.96022847
ATCGCGG32150.055.96006440
ATCTCGT137400.055.4584640
ACGTCTG428750.055.32581315
CATCTCG155700.055.281239
TCATCGC107700.054.8291538
TGCCGTC109900.054.5854848
ACACGTC435850.054.4957713
TCGCGTT27450.054.3204541
Produced by FastQC (version 0.11.2)