FastQCFastQC Report
Fri 27 Jul 2018
HWLMWBGX5_n01_mb109.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWLMWBGX5_n01_mb109.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31792201
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC2278710.716751256070632TruSeq Adapter, Index 6 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGTA271800.063.8618541
CTCGTAT269750.063.7499942
TCGTATG268600.063.59249543
CGTATGC272500.063.1436844
ATGCCGT268550.062.8836247
CGCCAAT290600.062.80982633
TATCTCG282800.062.43155339
TATGCCG279300.061.45432746
ACGCCAA299100.061.2343732
TGCCGTC273850.061.14239548
ATCTCGT289200.061.085140
GCCGTCT268500.060.43147749
CCGTCTT271400.060.3152450
GTCACGC306800.059.88141329
TCACGCC313250.058.47062330
CGTCTTC272550.058.1986951
CACGCCA321150.056.95375431
AGTCACG326900.056.68103828
GTATGCC315500.054.91362445
GCCAATA350050.052.29216834
Produced by FastQC (version 0.11.2)