FastQCFastQC Report
Fri 27 Jul 2018
HWLMWBGX5_n01_mb108.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWLMWBGX5_n01_mb108.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31120851
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGTCGTATGC1128080.36248366087418366TruSeq Adapter, Index 5 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC1062720.34148166449561423TruSeq Adapter, Index 5 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC291550.064.2875344
TCGTATG255150.064.2281343
ATGCCGT270150.063.3271347
GTCGTAT144600.062.9839342
TATGCCG301900.062.41807646
TCTCGTA138100.062.17207341
TGCCGTC270750.062.11385748
CTCGTAT137750.061.94879542
TCGCGTA21600.061.9014941
GATGTCG174500.061.6412139
TGTCGTA148750.061.57979241
CCGTCTT262000.061.3566350
GCCGTCT249700.060.97293549
CGCGTAT21550.060.5832242
CGTCTGA509100.060.4932416
ACGTCTG516300.059.72362515
CGTCTTC246750.059.36118351
ATGTCGT170350.058.9293940
ACACGTC525950.058.7536913
GATCTCG161100.058.70753539
Produced by FastQC (version 0.11.2)