FastQCFastQC Report
Fri 27 Jul 2018
HWLMWBGX5_n01_mb107.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWLMWBGX5_n01_mb107.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29387780
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC9978783.395554206544353TruSeq Adapter, Index 4 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT1149400.066.2127542
TCTCGTA1147100.066.1506841
TCGTATG1185000.066.0784243
ATGCCGT1169850.066.0477147
CGTATGC1186650.065.9839644
TGCCGTC1162050.065.8673548
TATGCCG1183950.065.8616746
GCCGTCT1137600.065.7906149
CCGTCTT1143750.065.7183850
AATCTCG1155100.065.7036339
ATCTCGT1163550.065.3600740
CGTCTTC1129100.065.3249551
GTATGCC1227100.063.92165845
ACCAATC1216550.062.41114436
CGTCTGA1323800.062.2237716
GACCAAT1270550.061.99373635
ACGTCTG1335850.061.6885815
GCTTGAA1176250.061.5489659
CCAATCT1236350.061.3508237
CAATCTC1244400.060.992638
Produced by FastQC (version 0.11.2)