FastQCFastQC Report
Fri 27 Jul 2018
HWLMWBGX5_n01_mb106.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWLMWBGX5_n01_mb106.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34761882
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC1545480.44459042810167754TruSeq Adapter, Index 2 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCGCGTATGC477910.1374810489259471TruSeq Adapter, Index 2 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGCG140900.065.16151439
CGATGTA580200.065.1571634
TCGCGTA76150.065.0397941
ACCGATG587350.064.58478532
CCGATGT586800.064.5146333
CACCGAT591100.064.310931
CGCGTAT77050.063.8254442
ATCGCGT108800.063.2486140
GTATCGC150350.062.79039838
TCTCGTA212200.062.7627441
TGTATCG249650.062.577137
CTCGTAT210650.062.54293442
GTCACCG609300.062.5340929
TCACCGA610350.062.44497730
CGTATGC233350.062.29228644
TATCTCG271700.062.23737339
TCGTATG194350.062.02509743
ATGCCGT222000.061.171247
TATGCCG261900.060.91320446
GTATCGG99800.060.18696638
Produced by FastQC (version 0.11.2)