FastQCFastQC Report
Tue 3 Jul 2018
HWLKJBGX5_n01_mycscreens2_5_bc_bc9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWLKJBGX5_n01_mycscreens2_5_bc_bc9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12283984
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGATCG1828700.069.958881
TCGATTC2624750.069.849512
ATCGATT2623600.069.845661
TCGATAA4058500.069.807563
TAACAAT1873700.069.726361
GATAACG4057700.069.71525
ATAACGC4054900.069.7092366
TAACGCA4052600.069.695557
CGATAAC4064600.069.6617364
AACGCAT4055150.069.622368
ATTCCTT2632050.069.604295
TCGATAG1840050.069.572195
ACGCATT4058450.069.567569
TCGATAC1762500.069.540634
CGATTCC2635100.069.488823
TTGGTTC2631250.069.396410
CGCATTT4065600.069.3662910
TTCCTTG2641150.069.3568046
GGTTCTT2631900.069.3209412
GATTCCT2644450.069.310434
Produced by FastQC (version 0.11.2)