FastQCFastQC Report
Tue 3 Jul 2018
HWLKJBGX5_n01_mycscreens2_5_bc_bc24.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWLKJBGX5_n01_mycscreens2_5_bc_bc24.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13017769
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGCGCGCGGCGCGGCGC154097611.83748152237146No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGCGCGCGGCGCGGCGCG136616610.494624693371039No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGCGCGCGGCGC135947310.443210353479156No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGCGCGCGGCG135841610.435090682589312No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGCGCGCGGCGCGGCGCGG11924449.160125671303584No Hit
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGCCAATACGGACCGGA3336332.5629045960179506No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGCCAATACGGA3007182.310057890872084No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGCCAATACGG2977712.2874196031593432No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGCCAATACGGACCGGAT2957112.271595079003169No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGCCAATACGGACCGGATT2722962.0917255483639328No Hit
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGCTTTGGGGATCTCAA2201451.6911115875539042No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGCTTTGGGGA1997431.5343873439450337No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGCTTTGGGGAT1985781.5254380378081682No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGCTTTGGGGATCTCAAG1950321.4981983471975882No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGCTTTGGGGATCTCAAGA1794771.3787078262027848No Hit
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGGTAGCGCACGATATT1670981.2836147269167244No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGGTAGCGCACG1503111.1546602186595876No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGGTAGCGCAC1490311.1448275046207994No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGGTAGCGCACGATATTA1481031.137698786942678No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGGTAGCGCACGATATTAG1350071.0370978314333277No Hit
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGGATTCTGAGGGAAAT904900.695126791695259No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGGATTCTGAG817870.628272018039343No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGGATTCTGAGG814110.625383658290449No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGGATTCTGAGGGAAATT791530.6080381361813995No Hit
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGCTGAAGGTTCCAGGT791370.6079152272559146No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGGATTCTGAGGGAAATTG725780.5575302496149686No Hit
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGGAGACGGACGTCTCT711770.5467680368272013No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGCTGAAGGTT709590.5450934027174703No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGCTGAAGGTTC707180.5432420870273547No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGCTGAAGGTTCCAGGTC702570.5397007736118224No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGGAGACGGAC641490.4927802913079806No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGGAGACGGACG640750.49221183752761316No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGGAGACGGACGTCTCTG637760.4899149769826151No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGCTGAAGGTTCCAGGTCA636570.4890008418493215No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGGAGACGGACGTCTCTGT563640.432977417251758No Hit
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGCTCCGCGCTTCTGCT217540.1671100478123402No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGCTCCGCGCTTCTGCTT194960.14976452570329063No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGCTCCGCGCT190190.14610030336227353No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGCTCCGCGCTT190100.14603116709168829No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGCTCCGCGCTTCTGCTTC168920.12976109808063116No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCACC700.070.007857
GTCGCAC1600.070.00656
GTCGTAT850.070.00656
TCGTTAG1500.070.006235
TCGACAC550.070.0054244
TCGGTAC500.070.0054244
TACCGTA455.456968E-1270.005162
TAAGTCG1950.070.005153
ATATCGC150.002224772270.005152
GGTTTTC900.070.002212
TTTTCTT900.070.00165614
ATTTTTG550.070.0003115
ACTCGAT353.7180143E-969.999511
ACGTTCG2250.069.9995041
ACGCTCG600.069.9995041
ACGGTCG550.069.9995041
ACGAACG1350.069.9995041
CGTTCGC150.002225619769.9984334
ACGATCG2565950.069.964041
TAACAAT2248100.069.9528051
Produced by FastQC (version 0.11.2)