FastQCFastQC Report
Tue 3 Jul 2018
HWLKJBGX5_n01_mycscreens2_5_bc_bc23.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWLKJBGX5_n01_mycscreens2_5_bc_bc23.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13998942
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGCGCGCGGCGCGGCGC158208611.301468353822738No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGCGCGCGGCGC140769810.055745641349182No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGCGCGCGGCGCGGCGCG13534979.668566381659414No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGCGCGCGGCG13450289.608068952639421No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGCGCGCGGCGCGGCGCGG13031079.308610607858794No Hit
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGCCAATACGGACCGGA3410892.436534132365146No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGCCAATACGGA3087652.205630968397469No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGCCAATACGGACCGGAT2958002.1130168265573213No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGCCAATACGG2955502.1112309773124283No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGCCAATACGGACCGGATT2955412.111166686739612No Hit
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGGCCGCCACTGGAGGG1807551.2912047210424902No Hit
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGGTAGCGCACGATATT1721221.2295357749178475No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGGCCGCCACTG1627041.1622592621642407No Hit
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGCTTTGGGGATCTCAA1600091.1430078073042949No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGGTAGCGCACG1571651.122691986294393No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGGCCGCCACTGGAGGGC1559511.1140199023611927No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGGCCGCCACT1554481.110426773680468No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGGCCGCCACTGGAGGGCG1514751.0820460574806297No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGGTAGCGCACGATATTAG1497311.0695879731482565No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGGTAGCGCAC1494121.067309229511773No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGGTAGCGCACGATATTA1490571.064773323584025No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGCTTTGGGGAT1455551.039757147361565No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGCTTTGGGGA1397940.9986040373622521No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGCTTTGGGGATCTCAAGA1389940.9928893197785946No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGCTTTGGGGATCTCAAG1364360.9746165103048501No Hit
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGGAGACGGACGTCTCT887170.6337407498366662No Hit
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGCTGAAGGTTCCAGGT863200.6166180272766328No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGGAGACGGACG818760.5848727710994159No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGCTGAAGGTTC789740.5641426330646988No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGGAGACGGACGTCTCTG776780.5548847905791737No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGGAGACGGAC775600.5540418697355843No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGGAGACGGACGTCTCTGT759030.542205260940434No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGCTGAAGGTTCCAGGTC753380.5381692416469759No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGCTGAAGGTT752300.5373977547731821No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGCTGAAGGTTCCAGGTCA750510.5361190867138388No Hit
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGGATTCTGAGGGAAAT717370.5124458691235381No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGGATTCTGAGG647200.4623206525178831No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGGATTCTGAG629480.44966255307008196No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGGATTCTGAGGGAAATTG611980.4371616083558315No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGGATTCTGAGGGAAATT605330.4324112493644163No Hit
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGCTCCGCGCTTCTGCT326250.23305332645852808No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGCTCCGCGCTT295030.2107516410883051No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGCTCCGCGCTTCTGCTT280830.2006080173773132No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGCTCCGCGCT278510.19895074927805256No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGCTCCGCGCTTCTGCTTC277700.19837213412270727No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCATT2500.070.0090567
TCGTATC1000.070.0090567
TCGGTAC301.0244912E-770.006314
AAGTCGC1650.070.006314
TACGTCG301.024564E-770.005563
ATCGTAC2050.070.0055543
AACGTTA150.002224756270.0053146
GCTTGCG150.002224756270.0053146
TGCTCGT252.8416162E-670.004351
ACGATTA252.842702E-669.999811
GCGATAA150.00222576569.9973153
ACGATCG2667600.069.9683151
TAACAAT2600700.069.9580841
ATACTGT2641900.069.915021
TACTGTA2641400.069.892922
ATAGGTC3096750.069.8540952
ACTGTAT2644350.069.81233
AACAATG2614900.069.794062
TAGGTCG3097350.069.791973
CTGTATC2645350.069.7668154
Produced by FastQC (version 0.11.2)