FastQCFastQC Report
Tue 3 Jul 2018
HWLKJBGX5_n01_mycscreens2_5_bc_bc21.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWLKJBGX5_n01_mycscreens2_5_bc_bc21.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12905999
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGCGCGCGGCGCGGCGC11319988.771099393390624No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGCGCGCGGCGCGGCGCG10709258.29788534773635No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGCGCGCGGCGC10313447.991198511637883No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGCGCGCGGCGCGGCGCGG9891647.664373753631935No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGCGCGCGGCG9884827.659089389360715No Hit
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGCCAATACGGACCGGA3606142.7941579725831374No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGCCAATACGGACCGGAT3530892.735851753901422No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGCCAATACGGA3428902.6568264882090875No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGCCAATACGGACCGGATT3399802.634278834207255No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGCCAATACGG3313892.5677128907262428No Hit
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGGTAGCGCACGATATT2019511.5647839427230703No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGGTAGCGCACGATATTA1965481.522919690292863No Hit
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGCTTTGGGGATCTCAA1925791.4921665498346932No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGGTAGCGCACG1918361.4864095371462527No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGCTTTGGGGATCTCAAG1899221.4715792245141195No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGGTAGCGCACGATATTAG1886701.4618783094590353No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGCTTTGGGGAT1857611.439338403791911No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGGTAGCGCAC1839501.4253061696347566No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGCTTTGGGGATCTCAAGA1829581.4176198216038913No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGCTTTGGGGA1804911.3985046798779388No Hit
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGGATTCTGAGGGAAAT1628541.2618473006235318No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGGATTCTGAGGGAAATT1562091.2103596164853259No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGGATTCTGAGG1538881.1923757316268193No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGGATTCTGAGGGAAATTG1511311.1710135728353923No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGGATTCTGAG1495521.1587789523306178No Hit
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGCTGAAGGTTCCAGGT1165540.9030994036184258No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGCTGAAGGTTCCAGGTC1132510.8775066540761394No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGCTGAAGGTTC1098310.8510073493729543No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGCTGAAGGTTCCAGGTCA1088770.8436154380610134No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGCTGAAGGTT1062750.8234542711494088No Hit
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGGAGACGGACGTCTCT918070.7113513645863447No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGGAGACGGACGTCTCTG881320.6828762345324837No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGGAGACGGACG847670.656803088238268No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGGAGACGGAC820180.6355029161245093No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGGAGACGGACGTCTCTGT813630.6304277568904196No Hit
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGCTCCGCGCTTCTGCT231870.1796606368867687No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGCTCCGCGCTTCTGCTT225210.17450024597088531No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGCTCCGCGCTT212600.16472959590342445No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGCTCCGCGCTTCTGCTTC205570.15928251660332532No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGCTCCGCGCT205110.15892609320673276No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCTTC1000.070.005857
TCGACTT150.002224683870.005857
TCGTATC950.070.0058447
GTCCGCA207.928708E-570.005316
TCGATCA1050.070.004774
TCGTTCC207.929621E-570.0036850
ATATCGC207.929773E-570.003412
GTTTTCT1200.070.0001513
TTTTTGT500.069.99934416
ACGTTCG2000.069.99881
TCTTTTT150.002225641269.9982654
ACGATCG2431450.069.965691
TAACAAT2449200.069.948791
TACTGTA2591700.069.896722
ATACTGT2593350.069.8948751
ATAGGTC2714100.069.839632
ACTGTAT2594800.069.7986453
TAGGTCG2713150.069.788243
CTGTATC2594600.069.782174
AACAATG2464600.069.7562942
Produced by FastQC (version 0.11.2)